Nanopore Biosensor for Label-Free and Real-Time Detection of Anthrax Lethal Factor | Zendy

Liang Wang | Zendy; Yujing Han | Zendy; Shuo Zhou | Zendy; Guihua Wang | Zendy; Xiyun Guan | Zendy

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Nanopore Biosensor for Label-Free and Real-Time Detection of Anthrax Lethal Factor

Author(s) -

Liang Wang,

Yujing Han,

Shuo Zhou,

Guihua Wang,

Xiyun Guan

Publication year - 2014

Publication title -

acs applied materials and interfaces

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.535

H-Index - 228

eISSN - 1944-8252

pISSN - 1944-8244

DOI - 10.1021/am500749p

Subject(s) - nanopore , bacillus anthracis , biosensor , anthrax toxin , dna , nanotechnology , materials science , nanopore sequencing , computational biology , biology , dna sequencing , genetics , gene , bacteria , recombinant dna , fusion protein

We report a label-free real-time nanopore sensing method for the detection of anthrax lethal factor, a component of the anthrax toxin, by using a complementary single-stranded DNA as a molecular probe. The method is rapid and sensitive: sub-nanomolar concentrations of the target anthrax lethal factor DNA could be detected in ∼1 min. Further, our method is selective, which can differentiate the target DNA from other single-stranded DNA molecules at the single-base resolution. This sequence-specific detection approach should find useful application in the development of nanopore sensors for the detection of other pathogens.

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