In Vitro Selection of Peptides and Proteins—Advantages of mRNA Display | Zendy

Matilda Newton | Zendy; Yari Cabezas-Perusse | Zendy; Cher Ling Tong | Zendy; Burckhard Seelig | Zendy

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In Vitro Selection of Peptides and Proteins—Advantages of mRNA Display

Author(s) -

Matilda Newton,

Yari Cabezas-Perusse,

Cher Ling Tong,

Burckhard Seelig

Publication year - 2019

Publication title -

acs synthetic biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.156

H-Index - 66

ISSN - 2161-5063

DOI - 10.1021/acssynbio.9b00419

Subject(s) - phage display , messenger rna , directed molecular evolution , computational biology , selection (genetic algorithm) , directed evolution , in vitro , biology , protein engineering , genetics , computer science , biochemistry , enzyme , gene , peptide , artificial intelligence , mutant

mRNA display is a robust in vitro selection technique that allows the selection of peptides and proteins with desired functions from libraries of trillions of variants. mRNA display relies upon a covalent linkage between a protein and its encoding mRNA molecule; the power of the technique stems from the stability of this link, and the large degree of control over experimental conditions afforded to the researcher. This article describes the major advantages that make mRNA display the method of choice among comparable in vivo and in vitro methods, including cell-surface display, phage display, and ribosomal display. We also describe innovative techniques that harness mRNA display for directed evolution, protein engineering, and drug discovery.

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