A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System | Zendy

Barbora Lavickova | Zendy; Sebastian J. Maerkl | Zendy

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A Simple, Robust, and Low-Cost Method To Produce the PURE Cell-Free System

Author(s) -

Barbora Lavickova,

Sebastian J. Maerkl

Publication year - 2019

Publication title -

acs synthetic biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.156

H-Index - 66

ISSN - 2161-5063

DOI - 10.1021/acssynbio.8b00427

Subject(s) - cell free protein synthesis , cell free system , yield (engineering) , simple (philosophy) , translation system , synthetic biology , computer science , biological system , biochemical engineering , process engineering , chemistry , protein biosynthesis , combinatorial chemistry , computational biology , biology , biochemistry , engineering , materials science , in vitro , philosophy , epistemology , metallurgy

We demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription-translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 μg/mL at a cost of 0.09 USD/μL, leading to a 14-fold improvement in cost normalized protein synthesis yield over existing PURE systems. The one-pot method makes the PURE system easy to generate and allows it to be readily optimized and modified.

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