Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii | Zendy

Pierre Crozet | Zendy; Francisco J. Navarro | Zendy; Felix Willmund | Zendy; Payam Mehrshahi | Zendy; Kamil Bakowski | Zendy; Kyle J. Lauersen | Zendy; Maria-Esther Pérez-Pérez | Zendy; Pascaline Auroy | Zendy; Aleix Gorchs Rovira | Zendy; Susana Sauret-Gueto | Zendy; Justus Niemeyer | Zendy; Benjamin Spaniol | Zendy; Jasmine Theis | Zendy; Raphael Trösch | Zendy; Lisa Désirée Westrich | Zendy; Konstantinos Vavitsas | Zendy; Thomas Baier | Zendy; Wolfgang Hübner | Zendy; Félix de Carpentier | Zendy; Mathieu Cassarini | Zendy; Antoine Da | Zendy; Julien Henri | Zendy; Christophe Marchand | Zendy; Marcello de Mia | Zendy; Kevin Sarkissian | Zendy; David C. Baulcombe | Zendy; Gilles Peltier | Zendy; José L. Crespo | Zendy; Olaf Kruse | Zendy; Poul Erik Jensen | Zendy; Michael Schroda | Zendy; Alison G. Smith | Zendy; Stéphane D. Lemaire | Zendy

Open Access

Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii

Author(s) -

Pierre Crozet,

Francisco J. Navarro,

Felix Willmund,

Payam Mehrshahi,

Kamil Bakowski,

Kyle J. Lauersen,

Maria-Esther Pérez-Pérez,

Pascaline Auroy,

Aleix Gorchs Rovira,

Susana Sauret-Gueto,

Justus Niemeyer,

Benjamin Spaniol,

Jasmine Theis,

Raphael Trösch,

Lisa Désirée Westrich,

Konstantinos Vavitsas,

Thomas Baier,

Wolfgang Hübner,

Félix de Carpentier,

Mathieu Cassarini,

Antoine Da,

Julien Henri,

Christophe Marchand,

Marcello de Mia,

Kevin Sarkissian,

David C. Baulcombe,

Gilles Peltier,

José L. Crespo,

Olaf Kruse,

Poul Erik Jensen,

Michael Schroda,

Alison G. Smith,

Stéphane D. Lemaire

Publication year - 2018

Publication title -

acs synthetic biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.156

H-Index - 66

ISSN - 2161-5063

DOI - 10.1021/acssynbio.8b00251

Subject(s) - chlamydomonas reinhardtii , synthetic biology , chlamydomonas , biology , modular design , computational biology , chassis , cloning (programming) , microbiology and biotechnology , workflow , systems biology , biochemical engineering , computer science , gene , genetics , engineering , programming language , structural engineering , database , mutant

Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, to realize their potential as green cell factories, a major challenge is to make microalgae easier to engineer. A promising approach for rapid and predictable genetic manipulation is to use standardized synthetic biology tools and workflows. To this end we have developed a Modular Cloning toolkit for the green microalga Chlamydomonas reinhardtii. It is based on Golden Gate cloning with standard syntax, and comprises 119 openly distributed genetic parts, most of which have been functionally validated in several strains. It contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, and introns cloned in various positions to allow maximum modularity. The toolkit enables rapid building of engineered cells for both fundamental research and algal biotechnology. This work will make Chlamydomonas the next chassis for sustainable synthetic biology.

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