In Vitro Selection for Small-Molecule-Triggered Strand Displacement and Riboswitch Activity | Zendy

Laura Martini | Zendy; Adam J. Meyer | Zendy; Jared W. Ellefson | Zendy; John N. Milligan | Zendy; Michele Forlin | Zendy; Andrew D. Ellington | Zendy; Sheref S. Mansy | Zendy

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In Vitro Selection for Small-Molecule-Triggered Strand Displacement and Riboswitch Activity

Author(s) -

Laura Martini,

Adam J. Meyer,

Jared W. Ellefson,

John N. Milligan,

Michele Forlin,

Andrew D. Ellington,

Sheref S. Mansy

Publication year - 2015

Publication title -

acs synthetic biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.156

H-Index - 66

ISSN - 2161-5063

DOI - 10.1021/acssynbio.5b00054

Subject(s) - riboswitch , rna , nucleic acid , dna , small molecule , systematic evolution of ligands by exponential enrichment , in vitro , chemistry , duplex (building) , biology , biochemistry , nucleic acid structure , computational biology , non coding rna , microbiology and biotechnology , gene

An in vitro selection method for ligand-responsive RNA sensors was developed that exploited strand displacement reactions. The RNA library was based on the thiamine pyrophosphate (TPP) riboswitch, and RNA sequences capable of hybridizing to a target duplex DNA in a TPP regulated manner were identified. After three rounds of selection, RNA molecules that mediated a strand exchange reaction upon TPP binding were enriched. The enriched sequences also showed riboswitch activity. Our results demonstrated that small-molecule-responsive nucleic acid sensors can be selected to control the activity of target nucleic acid circuitry.

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