A Dual-Purpose Real-Time Indicator and Transcriptional Integrator for Calcium Detection in Living Cells | Zendy

Elbegduuren Erdenee | Zendy; Alice Y. Ting | Zendy

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A Dual-Purpose Real-Time Indicator and Transcriptional Integrator for Calcium Detection in Living Cells

Author(s) -

Elbegduuren Erdenee,

Alice Y. Ting

Publication year - 2022

Publication title -

acs synthetic biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.156

H-Index - 66

ISSN - 2161-5063

DOI - 10.1021/acssynbio.1c00597

Subject(s) - integrator , dual (grammatical number) , calcium , computational biology , chemistry , computer science , biology , biological system , telecommunications , organic chemistry , bandwidth (computing) , art , literature

Calcium is a ubiquitous second messenger in eukaryotes, correlated with neuronal activity and T-cell activation among other processes. Real-time calcium indicators such as GCaMP have recently been complemented by newer calcium integrators that convert transient calcium activity into stable gene expression. Here we introduce LuCID, a dual-purpose real-time calcium indicator and transcriptional calcium integrator that combines the benefits of both calcium detection technologies. We show that the calcium-dependent split luciferase component of LuCID provides a real-time bioluminescence readout of calcium dynamics in cells, while the GI/FKF1 split GAL4 component of LuCID converts calcium-generated bioluminescence into stable gene expression. We also show that LuCID's modular design enables it to read out other cellular events such as protein-protein interactions. LuCID adds to the arsenal of tools for studying cells and cell populations that utilize calcium for signaling.

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