Open AccessMultistep Fractionation of Microalgal Biomolecules Using Selective Aqueous Two-Phase Systems
Author(s) -
Catalina A. Suarez Ruiz,
Jennifer Kwaijtaal,
Oriol Cabau Peinado,
C. van den Berg,
René H. Wijffels,
Michel H. M. Eppink
Publication year - 2020
Publication title -
acs sustainable chemistry and engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.878
H-Index - 109
ISSN - 2168-0485
DOI - 10.1021/acssuschemeng.9b06379
Subject(s) - fractionation , chemistry , aqueous solution , polypropylene glycol , biomolecule , chromatography , polymer , phase (matter) , extraction (chemistry) , ionic liquid , aqueous two phase system , foam fractionation , pigment , starch , chemical engineering , organic chemistry , biochemistry , polyethylene glycol , catalysis , pulmonary surfactant , engineering
We aim to develop liquid–liquid extraction processes for the fractionation of microalgal components (proteins, pigments, lipids, and carbohydrates). The partitioning behavior of microalgal pigments and proteins in aqueous two-phase systems (ATPS) composed of the polymer polypropylene glycol with molecular weight 400 (PPG 400) + various cholinium based-ionic liquids was studied. A process for fractionation of multiple components from disrupted Neochloris oleoabundans was developed and evaluated. Results show that cholinium dihydrogen phosphate (Ch DHp) allows the fractionation of pigments in the PPG 400-rich phase and proteins in the Ch DHp-rich phase with high selectivity. It was demonstrated that a multiproduct approach can fractionate free glucose, and proteins in the ionic liquid-rich phase, pigments in the polymer-rich phase, while starch and lipids are recovered at the interface.
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