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Uranium is one of the most common radioactive contaminants in the environment. As a major nuclear material in production, environmental samples (like soil and groundwater) can provide signatures on uranium production activity inside the facility. Thus, developing a new and portable analytical technology for uranium in aqueous media is significant not only for environmental monitoring, but also for nonproliferation. In this work, a label-free method for the detection of uranyl (UO 2  2+ ) ions is developed by monitoring the translocation of a peptide probe in a nanopore. Based on the difference in the number of peptide events in the absence and presence of uranyl ions, nanomolar concentration of UO 2  2+ ions could be detected in minutes. The method is highly selective; micromolar concentrations of Cd 2+ , Cu 2+ , Zn 2+ , Ni 2+ , Pb 2+ , Hg 2+ , Th 4+ , Mg 2+ , and Ca 2+ would not interfere with the detection of UO 2  2+ ions. In addition, simulated water samples were successfully analyzed.

Peptide-Mediated Nanopore Detection of Uranyl Ions in Aqueous Media