Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout | Zendy

Bo Tian | Zendy; Jing Ma | Zendy; Teresa Zardán Gómez de la Torre | Zendy; Ádám Bálint | Zendy; Marco Donolato | Zendy; Mikkel Fougt Hansen | Zendy; Peter Svedlindh | Zendy; Mattias Strömberg | Zendy

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Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout

Author(s) -

Bo Tian,

Jing Ma,

Teresa Zardán Gómez de la Torre,

Ádám Bálint,

Marco Donolato,

Mikkel Fougt Hansen,

Peter Svedlindh,

Mattias Strömberg

Publication year - 2016

Publication title -

acs sensors

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.055

H-Index - 57

ISSN - 2379-3694

DOI - 10.1021/acssensors.6b00379

Subject(s) - loop mediated isothermal amplification , amplicon , newcastle disease , biotinylation , reverse transcription loop mediated isothermal amplification , detection limit , microbiology and biotechnology , reverse transcriptase , rna , virus , virology , dna , biology , polymerase chain reaction , materials science , chemistry , chromatography , gene , genetics

Rapid and sensitive diagnostic methods based on isothermal amplification are ideal substitutes for PCR in out-of-lab settings. However, there are bottlenecks in terms of establishing low-cost and user-friendly readout methods for isothermal amplification schemes. Combining the high amplification efficiency of loop-mediated isothermal amplification (LAMP) with an optomagnetic nanoparticle-based readout system, we demonstrate ultrasensitive and rapid detection of Newcastle disease virus RNA. Biotinylated amplicons of LAMP and reverse transcription LAMP (RT-LAMP) bind to streptavidin-coated magnetic nanoparticles (MNPs) resulting in a dramatical increase in the hydrodynamic size of the MNPs. This increase was measured by an optomagnetic readout system and provided quantitative information on the amount of LAMP target sequence. Our assay resulted in a limit of detection of 10 aM of target sequence with a total assay time of 30 min. The assay has also been tested on clinical samples (vaccine and tissue specime...

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