Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization | Zendy

Julian Heep | Zendy; Paul H. K. Tuchecker | Zendy; Christoph Gebhardt | Zendy; M. Dürr | Zendy

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Combination of Thin-Layer Chromatography and Mass Spectrometry Using Cluster-Induced Desorption/Ionization

Author(s) -

Julian Heep,

Paul H. K. Tuchecker,

Christoph Gebhardt,

M. Dürr

Publication year - 2019

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.9b03060

Subject(s) - mass spectrometry , chromatography , direct electron ionization liquid chromatography–mass spectrometry interface , ionization , cluster (spacecraft) , chemistry , desorption , thin layer chromatography , analytical chemistry (journal) , materials science , ion , chemical ionization , computer science , adsorption , organic chemistry , programming language

Desorption/ionization induced by neutral clusters (DINeC) was employed for mass spectrometry (MS) of oligopeptides and lipids after separation by means of thin-layer chromatography (TLC). Clear and fragmentation-free spectra were obtained from the TLC plates without any further sample treatment. Mass-resolved chromatograms were deduced when scanning the TLC plates with the cluster beam along the direction of solvent movement. Using vancomycin and noncovalently bound complexes, the soft nature of DINeC was demonstrated also when used in combination with TLC. As a test application, TLC and DINeC-MS were employed to separate and detect different phospholipids obtained from egg yolk.

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