Colloidal Gold Immunochromatographic Assay for Rapid Detection of Carbadox and Cyadox in Chicken Breast | Zendy

Lingling Guo | Zendy; Xiaoling Wu | Zendy; Gang Cui | Zendy; Shanshan Song | Zendy; Hua Kuang | Zendy; Chuanlai Xu | Zendy

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Colloidal Gold Immunochromatographic Assay for Rapid Detection of Carbadox and Cyadox in Chicken Breast

Author(s) -

Lingling Guo,

Xiaoling Wu,

Gang Cui,

Shanshan Song,

Hua Kuang,

Chuanlai Xu

Publication year - 2020

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.9b02931

Subject(s) - detection limit , chicken breast , hapten , chromatography , chemistry , monoclonal antibody , colloidal gold , antibody , medicine , immunology , food science , nanotechnology , materials science , nanoparticle

Abused or misused carbadox (CBX) and cyadox (CYA) in animal feed may cause food safety concerns, threatening human health. Here, we describe the design of a novel hapten for preparation of a monoclonal antibody against CBX and CYA simultaneously. Using this antibody, colloidal gold immunochromatographic assay (GICA) was developed for screening of CBX and CYA residues in chicken breast. Under optimal conditions, semiquantitative analysis results were visible by eye, with a visual limit of detection of 8 μg/kg for CBX and CYA, and cut-off values of 20 μg/kg for CBX and 40 μg/kg for CYA in chicken breast. Quantitative analysis could be performed using a hand-held strip scanner, with a calculated limit of detection of 2.92 μg/kg for CBX and 2.68 μg/kg for CYA in chicken breast. Validated by liquid chromatography-MS/MS, the developed GICA provides a useful tool for rapid on-site CBX and CYA residue screening in chicken breast.

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