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Four rhodamine 6G-based chemosensors ( H   3   L1 - H   3   L4 ) are designed for selective detection of Al 3+ ion. They are characterized using various spectroscopic techniques and X-ray crystallography. All absorption and emission spectral studies have been performed in 10 mM N -(2-hydroxyethyl)piperazine- N '-ethanesulfonic acid (HEPES) buffer solution at pH 7.4 in H 2 O/MeOH (9:1, v/v) at 25 °C. In absorption spectra, chemosensors exhibit an intense band around 530 nm in the presence of Al 3+ ion. Chemosensors ( H   3   L1 - H   3   L4 ) are nonfluorescent when excited around 490 nm. The presence of Al 3+ ion enhances the emission intensity (555 nm) many times. The formation of complexes 1 - 4 is established with the aid of different spectroscopic techniques. The limit of detection value obtained in the nanomolar range confirms the high sensitivity of the probes toward Al 3+ ion. It has been observed that the presence of aliphatic spacers in the diamine part and different halogen substituents in the salicylaldehyde part strongly influences the selectivity of the chemosensors toward Al 3+ ion. The propensity of the chemosensors to identify intracellular Al 3+ ions in triple-negative human breast cancer cell line MDA-MB-468 by fluorescence imaging is also examined in this study.

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Development of Rhodamine 6G-Based Fluorescent Chemosensors for Al<sup>3+</sup>-Ion Detection: Effect of Ring Strain and Substituent in Enhancing Its Sensing Performance

Development of Rhodamine 6G-Based Fluorescent Chemosensors for Al3+-Ion Detection: Effect of Ring Strain and Substituent in Enhancing Its Sensing Performance

Development of Rhodamine 6G-Based Fluorescent Chemosensors for Al³⁺-Ion Detection: Effect of Ring Strain and Substituent in Enhancing Its Sensing Performance