Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy | Zendy

Noemí Molina | Zendy; Marvin Cnudde | Zendy; Juan Antonio Guadix | Zendy; José M. PérezPomares | Zendy; Cristian A. Strassert | Zendy; Yolanda Vida | Zendy; Ezequiel PérezInestrosa | Zendy

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Platinum-Doped Dendritic Structure as a Phosphorescent Label for Bacteria in Two-Photon Excitation Microscopy

Author(s) -

Noemí Molina,

Marvin Cnudde,

Juan Antonio Guadix,

José M. PérezPomares,

Cristian A. Strassert,

Yolanda Vida,

Ezequiel PérezInestrosa

Publication year - 2019

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.9b00639

Subject(s) - phosphorescence , microscopy , two photon excitation microscopy , fluorescence microscope , photochemistry , quenching (fluorescence) , moiety , materials science , platinum , fluorescence , chemistry , singlet oxygen , nanotechnology , oxygen , stereochemistry , optics , organic chemistry , catalysis , physics

Herein, we present a water-soluble dendritric Pt(II) complex as a phosphorescent label for bacterial cells. The dendritic moiety endows the Pt(II) complex with unique properties such as water solubility, shielding from quenching by dioxygen, and binding to bacterial surfaces. The new biosensor was employed for two-photon excitation microscopy, and the binding was confirmed by electron microscopy, which demonstrates that such hybrid arrays can provide orthogonal yet complementary readouts.

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