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Bisulfite and sulfite (HSO 3  - /SO 3  2- ) are not only widely used toxic chemicals but also active anions with important biological functions. Hence, the development of new detection methods for HSO 3  - /SO 3  2- is important for environmental security and human health. In this paper, we report a symmetrical hemicyanine for the detection of HSO 3  - /SO 3  2- , SHC , which is constructed with p -diphthalaldehyde with trimethylbenzoindolium via condensation. The red fluorescent probe can fast respond to HSO 3  - /SO 3  2- (&lt;30 s) to give cyan fluorescence, and its sensing process is twice nucleophilic additions, which were observed from fluorescence response, initial ratiometric change, and subsequent turn-on increment; especially in a low-concentration level, the ratiometric fluorescence measurement can eliminate environmental interference. This probe can achieve a quantitative detection of HSO 3  - /SO 3  2- in a wide concentration range. Furthermore, the probe SHC is a mitochondria-specific probe for ratiometric fluorescent detection of HSO 3  - /SO 3  2- in living cells.

Two-Step Sensing, Colorimetric and Ratiometric Fluorescent Probe for Rapid Detection of Bisulfite in Aqueous Solutions and in Living Cells