Enhanced Intercellular Delivery of cRGD–siRNA Conjugates by an Additional Oligospermine Modification | Zendy

Kosuke Nakamoto | Zendy; Yukihiro Akao | Zendy; Yasuhiro Furuichi | Zendy; Yoshihito Ueno | Zendy

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Enhanced Intercellular Delivery of cRGD–siRNA Conjugates by an Additional Oligospermine Modification

Author(s) -

Kosuke Nakamoto,

Yukihiro Akao,

Yasuhiro Furuichi,

Yoshihito Ueno

Publication year - 2018

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.8b00850

Subject(s) - conjugate , small interfering rna , transfection , cationic polymerization , biophysics , gene silencing , cytotoxicity , intracellular , chemistry , rna , membrane permeability , peptide , gene delivery , membrane , combinatorial chemistry , microbiology and biotechnology , in vitro , biochemistry , biology , polymer chemistry , gene , mathematical analysis , mathematics

Small interfering RNA (siRNA), consisting a 21-mer duplex molecule, is often modified by conjugation with specific ligands to enhance its capacity for tissue-specific delivery. However, these attempts are hampered by the low permeability of negatively charged RNA molecules to enter the cell membrane. In this study, we designed and synthesized siRNA conjugates modified with cationic oligospermine and cyclic RGD (cRGD) to overcome the low-membrane permeability of siRNA. The siRNA conjugate, which contains 15 spermines and a cRGD peptide, showed sufficient gene-silencing activity at 250 nM final concentration without a transfection reagent. Under these conditions, the cationic oligospermine and cRGD-siRNA conjugate did not show any cytotoxicity.

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