In Situ Synthesis of Luminescent Au Nanoclusters on a Bacterial Template for Rapid Detection, Quantification, and Distinction of Kanamycin-Resistant Bacteria | Zendy

Upashi Goswami | Zendy; Amaresh Kumar Sahoo | Zendy; Arun Chattopadhyay | Zendy; Siddhartha Sankar Ghosh | Zendy

Open Access

In Situ Synthesis of Luminescent Au Nanoclusters on a Bacterial Template for Rapid Detection, Quantification, and Distinction of Kanamycin-Resistant Bacteria

Author(s) -

Upashi Goswami,

Amaresh Kumar Sahoo,

Arun Chattopadhyay,

Siddhartha Sankar Ghosh

Publication year - 2018

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.8b00504

Subject(s) - kanamycin , bacteria , nanoclusters , detection limit , colony forming unit , enumeration , microbiology and biotechnology , chemistry , gram , gram negative bacteria , in situ , luminescence , bacterial colony , biology , chromatography , nanotechnology , materials science , escherichia coli , biochemistry , optoelectronics , mathematics , genetics , organic chemistry , combinatorics , gene

Herein, we introduce a new facile method of luminescent gold nanocluster (Au NC) synthesis on the surface of bacteria for detection, counting, and strain differentiation. The limit of detection was 740 ± 14 colony-forming unit (CFU)/mL for the Gram-negative and was 634 ± 16 CFU/mL for the Gram-positive bacteria. Brief treatment with lysozyme could differentiate the Gram strains based on their luminescence intensities. The current method could also detect bacterial contaminants from water sources and kanamycin-resistant strains rapidly. This quick synthesis of Au NCs on a bacterial template attributes an easy and rapid method for enumeration and detection of bacterial contaminants and kanamycin-resistant strains.

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