From Live Cells to Caenorhabditis elegans: Selective Staining and Quantification of Lipid Structures Using a Fluorescent Hybrid Benzothiadiazole Derivative | Zendy

Alberto A. R. Mota | Zendy; José R. Corrêa | Zendy; Lorena P. de Andrade | Zendy; José Antônio Fagundes Assumpção | Zendy; Giovana A. de Souza Cintra | Zendy; Lúcio H. Freitas-Júnior | Zendy; Wender A. Silva | Zendy; Heibbe C. B. de Oliveira | Zendy; Brenno A. D. Neto | Zendy

Open Access

From Live Cells to Caenorhabditis elegans: Selective Staining and Quantification of Lipid Structures Using a Fluorescent Hybrid Benzothiadiazole Derivative

Author(s) -

Alberto A. R. Mota,

José R. Corrêa,

Lorena P. de Andrade,

José Antônio Fagundes Assumpção,

Giovana A. de Souza Cintra,

Lúcio H. Freitas-Júnior,

Wender A. Silva,

Heibbe C. B. de Oliveira,

Brenno A. D. Neto

Publication year - 2018

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.8b00434

Subject(s) - fluorescence , staining , caenorhabditis elegans , coumarin , derivative (finance) , bodipy , fluorescent staining , selectivity , fluorescence microscope , chemistry , biophysics , biology , biochemistry , gene , organic chemistry , optics , genetics , physics , financial economics , economics , catalysis

The current article describes the synthesis, characterization, and application of a designed hybrid fluorescent BTD-coumarin (2,1,3-benzothiadiazole-coumarin) derivative (named BTD-Lip ). The use of BTD-Lip for live-cells staining showed excellent results, and lipid droplets (LDs) could be selectively stained. When compared with the commercially available dye (BODIPY) for LD staining, it was noted that the designed hybrid fluorescence was capable of staining a considerable larger number of LDs in both live and fixed cells (ca. 40% more). The new dye was also tested on live Caenorhabditis elegans (complex model) and showed an impressive selectivity inside the worm, whereas the commercial dye showed no selectivity in the complex model.

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