Selective Photoinduced Antibacterial Activity of Amoxicillin-Coated Gold Nanoparticles: From One-Step Synthesis to in Vivo Cytocompatibility | Zendy

M. Jazmín Silvero C. | Zendy; Diamela María Rocca | Zendy; Emilce Artur de la Villarmois | Zendy; Kelsey Fournier | Zendy; Anabel E. Lanterna | Zendy; Mariela Fernanda Pérez | Zendy; M. C. Becerra | Zendy; J. C. Scaiano | Zendy

Open Access

Selective Photoinduced Antibacterial Activity of Amoxicillin-Coated Gold Nanoparticles: From One-Step Synthesis to in Vivo Cytocompatibility

Author(s) -

M. Jazmín Silvero C.,

Diamela María Rocca,

Emilce Artur de la Villarmois,

Kelsey Fournier,

Anabel E. Lanterna,

Mariela Fernanda Pérez,

M. C. Becerra,

J. C. Scaiano

Publication year - 2018

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.7b01779

Subject(s) - in vivo , biodistribution , biocompatibility , chemistry , amoxicillin , antibiotics , bacteria , staphylococcus aureus , antibacterial activity , antibacterial agent , fluorescence microscope , colloidal gold , nanoparticle , in vitro , combinatorial chemistry , nuclear chemistry , nanotechnology , materials science , fluorescence , biochemistry , biology , organic chemistry , genetics , physics , microbiology and biotechnology , quantum mechanics

Photoinduced antibacterial gold nanoparticles were developed as an alternative for the treatment of antibiotic-resistant bacteria. Thanks to the amoxicillin coating, they possess high in vivo stability, selectivity for the bacteria wall, a good renal clearance, and are completely nontoxic for eukaryotic cells at the bactericidal concentrations. A simple one-step synthesis of amoxi@AuNP is described at mild temperatures using the antibiotic as both reducing and stabilizing agent. Time-resolved fluorescence microscopy proved these novel nano-photosensitizers, with improved selectivity, are bactericidal but showing excellent biocompatibility toward eukaryotic cells at the same dose (1.5 μg/mL) when co-cultures are analyzed. Their stability in biological media, hemocompatibility, and photo-antibacterial effect against sensitive and antibiotic-resistant Staphylococcus aureus were evaluated in vitro, whereas toxicity, renal clearance, and biodistribution were studied in vivo in male Wistar rats. The use of these nanoparticles to treat antibiotic-resistant infections is promising given their high stability and cytocompatibility.

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