Streamlined Preparation of Immobilized Candida antarctica Lipase B | Zendy

Karim Engelmark Cassimjee | Zendy; Peter HendilForssell | Zendy; Alexey Volkov | Zendy; Anne Krog | Zendy; Jostein Malmo | Zendy; Trond Erik Vee Aune | Zendy; Wolfgang Knecht | Zendy; Iain Miskelly | Zendy; Thomas S. Moody | Zendy; Maria Svedendahl Humble | Zendy

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Streamlined Preparation of Immobilized Candida antarctica Lipase B

Author(s) -

Karim Engelmark Cassimjee,

Peter HendilForssell,

Alexey Volkov,

Anne Krog,

Jostein Malmo,

Trond Erik Vee Aune,

Wolfgang Knecht,

Iain Miskelly,

Thomas S. Moody,

Maria Svedendahl Humble

Publication year - 2017

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.7b01510

Subject(s) - candida antarctica , lipase , bioreactor , chemistry , escherichia coli , chromatography , biochemistry , enzyme , organic chemistry , gene

Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L -1 ) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/ Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.

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