Fluorescence Imaging of Disrupted Interfaces between Liquid-Ordered and Liquid-Disordered Domains by a Flavin-Labeled PNA Duplex | Zendy

Yoshimi Oka | Zendy; Hisae Shishino | Zendy

Open Access

Fluorescence Imaging of Disrupted Interfaces between Liquid-Ordered and Liquid-Disordered Domains by a Flavin-Labeled PNA Duplex

Author(s) -

Yoshimi Oka,

Hisae Shishino

Publication year - 2017

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.7b00581

Subject(s) - flavin group , peptide nucleic acid , vesicle , lipid microdomain , membrane , lipid raft , biophysics , fluorescence , fluorescence microscope , confocal , confocal microscopy , chemistry , nucleic acid , biology , biochemistry , microbiology and biotechnology , optics , physics , enzyme

Lipid rafts and membrane-active peptides are attracting attention because they help understand basic membrane functions. In addition, we focus on flavoproteins playing some physiological roles and explore the model compounds. In this study, we demonstrate that a new flavin probe, composed of palmitoylated peptide nucleic acid (PNA) and its complementary PNA labeled with flavin, targets the liquid-ordered (lo) microdomains and disrupts its interfaces to liquid-disordered (ld) microdomains of giant unilamellar vesicles and can be visualized by using confocal laser scanning microscopy. Surprisingly, as shown in time-lapse images, vesiculation and probe aggregations appear in the lo-ld interfaces, which leads to local disruption of the membrane. We discuss a possible interpretation of the data based on a comparison with control experiments.

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