Zein Microneedles for Transcutaneous Vaccine Delivery: Fabrication, Characterization, and in Vivo Evaluation Using Ovalbumin as the Model Antigen | Zendy

Shubhmita Bhatnagar | Zendy; Sumeet Rajesh Chawla | Zendy; Onkar P. Kulkarni | Zendy; Venkata Vamsi Krishna Venuganti | Zendy

Open Access

Zein Microneedles for Transcutaneous Vaccine Delivery: Fabrication, Characterization, and in Vivo Evaluation Using Ovalbumin as the Model Antigen

Author(s) -

Shubhmita Bhatnagar,

Sumeet Rajesh Chawla,

Onkar P. Kulkarni,

Venkata Vamsi Krishna Venuganti

Publication year - 2017

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.7b00343

Subject(s) - ovalbumin , antigen , penetration (warfare) , in vivo , antibody titer , materials science , antibody , biomedical engineering , medicine , immunology , titer , biology , microbiology and biotechnology , operations research , engineering

Transcutaneous antigen administration provides an alternative to invasive syringe injections. The objective of this study was to investigate the feasibility of fabrication and antigen delivery using microneedles made from corn protein, zein. Micromolding technique was used to cast cone-shaped zein microneedles (ZMNs). The insertion of ZMNs and the delivery of the model antigen, ovalbumin (OVA), into the skin was confirmed by histological examination and confocal microscopy. In addition, a significantly ( p < 0.05) lower bacterial skin penetration was observed after ZMN application compared with hypodermic syringe application. OVA coated on ZMNs was stable after storage under ambient and refrigerator conditions. Transcutaneous immunization studies showed significantly ( p < 0.001) greater antibody titers (total IgG, IgG1, and IgG2a) after the application of OVA-coated ZMNs and OVA intradermal injection compared with the control group. Taken together, antigen-coated ZMNs can be developed for transcutaneous vaccine delivery.

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