Open AccessUrinary Proteomic Characteristics of Hyperuricemia and Their Possible Links with the Occurrence of Its Concomitant Diseases
Author(s) -
Shuai Huo,
Hongxin Wang,
Meixia Yan,
Peng Xu,
Tingting Song,
Chuang Li,
Ruimin Tian,
Xiaoling Chen,
Kun Bao,
Ying Xie,
Ping Xu,
Weimin Zhu,
Fengsong Liu,
Wei Mao,
Chen Shao
Publication year - 2021
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.0c06229
Subject(s) - gout , hyperuricemia , proteome , urine , hyperlipidemia , purine metabolism , purine , diabetes mellitus , proteomics , uric acid , medicine , urinary system , concomitant , endocrinology , metabolomics , chemistry , biology , bioinformatics , biochemistry , enzyme , gene
Hyperuricemia (HUA), a chronic disease caused by metabolic disorders of purine, is often accompanied by other diseases such as gout, type 2 diabetes mellitus (T2DM), and hyperlipidemia. However, little is known about the relationship between HUA and these diseases on the protein level. We performed label-free liquid chromatography MS/MS spectrometry analysis of urine samples from 26 HUA patients and 25 healthy controls, attempting to establish the possible protein links between HUA and these diseases by profiling urine proteome. A total of 2119 proteins were characterized in sample proteomes. Among them, 11 were found decreased and 2 were found increased in HUA samples. Plausible pathways found by enrichment analysis of these differentially expressed proteins (DEPs) include the processes for insulin receptor recycling and lipid metabolism, suggesting potential links between HUA and T2DM and hyperlipidemia. The abundance changes of three key proteins (VATB1, CFAD, and APOC3) involved in these processes were validated by enzyme-linked immunosorbent assay (ELISA). In conclusion, our result provides proteomic evidence, for the first time, that the aberrant pathways enriched by described key DEPs are closely related to the incidence of HUA and its concomitant diseases.