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Lignocellulose is a promising raw material for the production of second-generation biofuels. In this study, the effects of acid-catalyzed liquid hot water (LHW) on pretreatment of corn stover (CS) for subsequent hydrolysis and conversion to ethanol were studied. The effects of reaction temperature, acid concentration, and residence time on glucose yield were evaluated using a response surface methodology. The optimal condition was 162.4 °C for 29.5 min with 0.45% v/v of sulfuric acid, leading to the maximum glucose yield of 91.05% from enzymatic hydrolysis of the cellulose-enriched fraction. Conversion of the solid fraction to ethanol by simultaneous saccharification and fermentation resulted in a theoretical ethanol yield of 93.91% based on digestible glucose. Scanning electron microscopy revealed disruption on the microstructure of the pretreated CS. Increases of crystallinity index and surface area of the pretreated biomass were observed along with alteration in the functional group profiles, as demonstrated by Fourier transform infrared spectroscopy. This work provides an insight into the effects of LHW on the enzymatic susceptibility and modification of the physicochemical properties of CS for further application on bioethanol production in biorefinery.

Efficiency of Catalytic Liquid Hot Water Pretreatment for Conversion of Corn Stover to Bioethanol