Short DNA Oligonucleotide as a Ag+ Binding Detector | Zendy

Fusheng Shen | Zendy; Song Mao | Zendy; Johnsi Mathivanan | Zendy; Ying Wu | Zendy; Arun Richard Chandrasekaran | Zendy; Hehua Liu | Zendy; Jianhua Gan | Zendy; Jia Sheng | Zendy

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Short DNA Oligonucleotide as a Ag⁺ Binding Detector

Author(s) -

Fusheng Shen,

Song Mao,

Johnsi Mathivanan,

Ying Wu,

Arun Richard Chandrasekaran,

Hehua Liu,

Jianhua Gan,

Jia Sheng

Publication year - 2020

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.0c03372

Subject(s) - molecular beacon , oligonucleotide , dna , fluorescence , base pair , detector , chemistry , biophysics , microbiology and biotechnology , crystallography , physics , biology , biochemistry , optics

Ag + has been known to mediate several natural metallo-base pairs. Based on the unique structural information of a short 8-mer DNA strand (5'-GCACGCGC-3') induced by Ag + , we constructed several fluorescent DNA beacons for the detection of Ag + according to the increase in the fluorescence emission on Ag + binding. This Ag + detection assay is quick, sensitive, and easy to adapt and can function in a wide range of temperatures from 5 to 65 °C.

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