Nanohydroxyapatite-, Gelatin-, and Acrylic Acid-Based Novel Dental Restorative Material

The aim of this study was to prepare a novel dental restorative material (NDRM) and to understand its cell viability behavior. The hydroxyapatite (HA) nanopowder was synthesized using a wet chemical precipitation method using calcium hydroxide and orthophosphoric acid as precursors. The as-prepared HA nanopowder was annealed at different temperatures to get a pure compound with a Ca/P ratio close to 1.67. The optimal temperature was found to be 600 °C, whereas at a higher temperature, HA starts decomposing into CaO. The preparation of NDRM was conducted in two steps. The first step comprises the preparation of HA nanopowder- and gelatin (G)-based film using microwave heating. In the second step, the homogenized mixture of the HA-G film was mixed with different amounts of acrylic acid to form a self-flowable NDRM paste. Further, both these materials (HA nanopowder and NDRM) were characterized using FTIR, XRD, and SEM-EDX analyses. The FTIR and XRD results show the peaks corresponding to natural bone apatite and therefore confirm the formation of HA. EDX results showed the presence of Ca and P in HA nanopowder and NDRM with Ca/P ratios of 1.79 and 1.63, respectively. Synthesized NDRM was also analyzed for its in vitro cytotoxic and reproductive viability potential against normal cells using MTT and clonogenic assay. The analysis showed significantly higher cellular viability on the treatment with NDRM when compared to HA nanopowder as well as no colony suppression by both materials was observed on the normal cell line (fR2) even after exposure for 24 h, indicating its nontoxicity. The synthesized NDRM therefore can be considered as a promising candidate for dental caries restoration applications.