C21 Fraction Refined from Marsdenia tenacissima-Induced Apoptosis is Enhanced by Suppression of Autophagy in Human Gastric Cell Lines | Zendy

Kaiqiang Li | Zendy; Ke Hao | Zendy; Yu Zhang | Zendy; Aibo Xu | Zendy; Qianni Wang | Zendy; Yaoqiang Du | Zendy; Lingling Wu | Zendy; Bingyu Chen | Zendy; Wei Zhang | Zendy; Zhen Wang | Zendy

Open Access

C21 Fraction Refined from Marsdenia tenacissima-Induced Apoptosis is Enhanced by Suppression of Autophagy in Human Gastric Cell Lines

Author(s) -

Kaiqiang Li,

Ke Hao,

Yu Zhang,

Aibo Xu,

Qianni Wang,

Yaoqiang Du,

Lingling Wu,

Bingyu Chen,

Wei Zhang,

Zhen Wang

Publication year - 2020

Publication title -

acs omega

Language(s) - English

Resource type - Journals

ISSN - 2470-1343

DOI - 10.1021/acsomega.0c02748

Subject(s) - apoptosis , autophagy , western blot , downregulation and upregulation , reactive oxygen species , protein kinase b , cancer , in vitro , chemistry , cell cycle , cancer cell , pharmacology , biology , cancer research , biochemistry , genetics , gene

C21 steroidal glycosides have been extensively reported for treating several types of cancer and are widely found in Marsdenia tenacissima . In this study, a C21 fraction was synthesized from M. tenacissima , and its anti-cancer potency was assessed against in vitro gastric cell lines BGC-823, SGC-7901, and AGS. Significant growth inhibition and cell cycle arrest were observed in C21 fraction-treated gastric cancer cells. The results of apoptotic staining techniques in C21 fraction-treated gastric cells were confirmed with excess reactive oxygen species generation. Moreover, SOD and H 2 O 2 levels were increased by C21 fraction, especially when combined with chloroquine (CQ). The apoptotic inducing potential of C21 fraction was also evidenced by upregulation of proapoptotic proteins cleaved-PARP and BAX and downregulation of antiapoptotic proteins Bcl-2 and p-AKT by western blot, especially in the presence of the autophagy inhibitor CQ. The results showed that the apoptosis of gastric cancer cells caused by C21 fraction was enhanced by inhibiting autophagy. The current findings reveal a new mechanism for the antitumor activity of C21 fraction on gastric cancer.

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