Synthesis, Characterization, and Biological Activity of Aminated Zymosan | Zendy

Geetha Venkatachalam | Zendy; Senthilkumar Arumugam | Zendy; Mukesh Doble | Zendy

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Synthesis, Characterization, and Biological Activity of Aminated Zymosan

Author(s) -

Geetha Venkatachalam,

Senthilkumar Arumugam,

Mukesh Doble

Publication year - 2020

Publication title -

acs omega

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.779

H-Index - 40

ISSN - 2470-1343

DOI - 10.1021/acsomega.0c01243

Subject(s) - chemistry , zymosan , macromolecule , biochemistry , fourier transform infrared spectroscopy , nuclear chemistry , in vitro , chemical engineering , engineering

Zymosan (ZM), a naturally occurring insoluble macromolecule obtained from the cell wall of Saccharomyces cerevisiae , is used as a functional food (as dietary fiber), phagocytic stimulus, and immune potentiator. The present study aimed to increase its solubility and evaluate its immunological application. ZM was converted into soluble 6-amino-6-deoxy-β-(1-3)-glucan of a molecular weight of 296 kDa by reduction. Detailed structural characterization of aminated ZM was determined by Fourier transform infrared spectroscopy and two-dimensional NMR analysis (2D, COSY, TOCSY, ROSEY, NOSEY, and HSQC). Aminated ZM was biocompatible with Raw 264.7 macrophage cell lines up to a concentration of 100 μg/mL. Rhodamine tagging revealed that the aminated ZM microparticles were found localized within the nucleus of Raw 264.7 cells. Both native and aminated ZM showed a similar expression pattern of inflammatory genes in Raw 264.7.

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