Fabrication of a Fibrous Metal–Organic Framework and Simultaneous Immobilization of Enzymes

A nanorod-like lanthanum metal-organic framework (LaMOF) was synthesized in aqueous solution by coordinating La(III) to the ligand 1,3,5-benzenetricarboxylic acid. The fibrous LaMOF was fabricated by splitting the nanorod-like LaMOF in a solution of d-amino acid oxidase, and the enzyme was immobilized simultaneously. Based on SEM and TEM images, STEM mapping, and spectra of XPS and FTIR, the mechanism of formation of the fibrous LaMOF and the distinct interfacial phenomena have been elucidated. The fabrication of the fibrous LaMOF and simultaneous immobilization of the enzyme were carried out in aqueous solutions at room temperature, without using any organic solvent. It is a clean and time- and energy-effective process. This work presents a distinct and clean methodology for the fabrication of the fibrous MOF. Potentially, the environmentally benign methodology can be extended to immobilize other enzymes.