Open AccessElectrochemical Detection of Genomic DNA Utilizing Recombinase Polymerase Amplification and Stem-Loop Probe
Author(s) -
Shirin Khaliliazar,
Liangqi Ouyang,
Andrew Piper,
Georgios Chondrogiannis,
Martin Hanze,
Anna Herland,
Mahiar Max Hamedi
Publication year - 2020
Publication title -
acs omega
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.779
H-Index - 40
ISSN - 2470-1343
DOI - 10.1021/acsomega.0c00341
Subject(s) - recombinase polymerase amplification , multiple displacement amplification , loop mediated isothermal amplification , recombinase , dna , applications of pcr , point of care , hybridization probe , computational biology , polymerase chain reaction , digital polymerase chain reaction , microbiology and biotechnology , biology , genetics , medicine , dna extraction , recombination , gene , nursing
Nucleic acid tests integrated into digital point-of-care (POC) diagnostic systems have great potential for the future of health care. However, current methods of DNA amplification and detection require bulky and expensive equipment, many steps, and long process times, which complicate their integration into POC devices. We have combined an isothermal DNA amplification method, recombinase polymerase amplification, with an electrochemical stem-loop (S-L) probe DNA detection technique. By combining these methods, we have created a system that is able to specifically amplify and detect as few as 10 copies/μL Staphylococcus epidermidis DNA with a total time to result of 70-75 min.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom