A Quiescent, Regeneration-Responsive Tissue Engineered Mesenchymal Stem Cell Bone Marrow Niche Model via Magnetic Levitation | Zendy

Emily E. Lewis | Zendy; Helen Wheadon | Zendy; Natasha Lewis | Zendy; Jingli Yang | Zendy; Margaret Mullin | Zendy; Andrew Hursthouse | Zendy; David Stirling | Zendy; Matthew J. Dalby | Zendy; Catherine C. Berry | Zendy

Open Access

A Quiescent, Regeneration-Responsive Tissue Engineered Mesenchymal Stem Cell Bone Marrow Niche Model via Magnetic Levitation

Author(s) -

Emily E. Lewis,

Helen Wheadon,

Natasha Lewis,

Jingli Yang,

Margaret Mullin,

Andrew Hursthouse,

David Stirling,

Matthew J. Dalby,

Catherine C. Berry

Publication year - 2016

Publication title -

acs nano

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.554

H-Index - 382

eISSN - 1936-086X

pISSN - 1936-0851

DOI - 10.1021/acsnano.6b02841

Subject(s) - stem cell , mesenchymal stem cell , microbiology and biotechnology , bone marrow , regeneration (biology) , clinical uses of mesenchymal stem cells , adult stem cell , biology , stem cell transplantation for articular cartilage repair , bone marrow stem cell , immunology , endothelial stem cell , in vitro , biochemistry

The bone marrow niche represents a specialized environment that regulates mesenchymal stem cell quiescence and self-renewal, yet fosters stem cell migration and differentiation upon demand. An in vitro model that embodies these features would open up the ability to perform detailed study of stem cell behavior. In this paper we present a simple bone marrow-like niche model, which comprises of nanomagnetically levitated stem cells cultured as multicellular spheroids within a type I collagen gel. The stem cells maintained are nestin positive and remain quiescent until regenerative demand is placed upon them. In response to coculture wounding, they migrate and appropriately differentiate upon engraftment. This tissue engineered regeneration-responsive bone marrow-like niche model will allow for greater understanding of stem cell response to injury and also facilitate as a testing platform for drug candidates in a multiwell plate format.

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