Open AccessDye-Sensitized Core/Active Shell Upconversion Nanoparticles for Optogenetics and Bioimaging Applications
Author(s) -
Xiang Wu,
Yuanwei Zhang,
Kendra Takle,
Osman Bilsel,
Zhanjun Li,
Hyungseok Lee,
Zijiao Zhang,
Dongsheng Li,
Wei Fan,
Chunying Duan,
Emory M. Chan,
Carlos Lois,
Yang Xiang,
Gang Han
Publication year - 2016
Publication title -
acs nano
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.554
H-Index - 382
eISSN - 1936-086X
pISSN - 1936-0851
DOI - 10.1021/acsnano.5b06383
Subject(s) - photon upconversion , materials science , ytterbium , nanotechnology , optogenetics , fluorescence , nanoparticle , absorption (acoustics) , biological imaging , doping , optoelectronics , photochemistry , chemistry , optics , physics , neuroscience , composite material , biology
Near-infrared (NIR) dye-sensitized upconversion nanoparticles (UCNPs) can broaden the absorption range and boost upconversion efficiency of UCNPs. Here, we achieved significantly enhanced upconversion luminescence in dye-sensitized core/active shell UCNPs via the doping of ytterbium ions (Yb(3+)) in the UCNP shell, which bridged the energy transfer from the dye to the UCNP core. As a result, we synergized the two most practical upconversion booster effectors (dye-sensitizing and core/shell enhancement) to amplify upconversion efficiency. We demonstrated two biomedical applications using these UCNPs. By using dye-sensitized core/active shell UCNP embedded poly(methyl methacrylate) polymer implantable systems, we successfully shifted the optogenetic neuron excitation window to a biocompatible and deep tissue penetrable 800 nm wavelength. Furthermore, UCNPs were water-solubilized with Pluronic F127 with high upconversion efficiency and can be imaged in a mouse model.