Open AccessSimultaneous Measurement of Striatal Dopamine and Hydrogen Peroxide Transients Associated with L-DOPA Induced Rotation in Hemiparkinsonian Rats
Author(s) -
Leslie R. Wilson,
Christie A. Lee,
Catherine F. Mason,
Sitora Khodjaniyazova,
Kevin Flores,
David C. Muddiman,
Leslie A. Sombers
Publication year - 2021
Publication title -
acs measurement science au
Language(s) - English
Resource type - Journals
ISSN - 2694-250X
DOI - 10.1021/acsmeasuresciau.1c00030
Subject(s) - neurochemical , dopamine , dyskinesia , chemistry , parkinson's disease , neuroscience , endocrinology , oxidative stress , medicine , psychology , disease
Parkinson's disease (PD) is a neurodegenerative disorder commonly treated with levodopa (L-DOPA), which eventually induces abnormal involuntary movements (AIMs). The neurochemical contributors to these dyskinesias are unknown; however, several lines of evidence indicate an interplay of dopamine (DA) and oxidative stress. Here, DA and hydrogen peroxide (H 2 O 2 ) were simultaneously monitored at discrete recording sites in the dorsal striata of hemiparkinsonian rats using fast-scan cyclic voltammetry. Mass spectrometry imaging validated the lesions. Hemiparkinsonian rats exhibited classic L-DOPA-induced AIMs and rotations as well as increased DA and H 2 O 2 tone over saline controls after 1 week of treatment. By week 3, DA tone remained elevated beyond that of controls, but H 2 O 2 tone was largely normalized. At this time point, rapid chemical transients were time-locked with spontaneous bouts of rotation. Striatal H 2 O 2 rapidly increased with the initiation of contraversive rotational behaviors in lesioned L-DOPA animals, in both hemispheres. DA signals simultaneously decreased with rotation onset. The results support a role for these striatal neuromodulators in the adaptive changes that occur with L-DOPA treatment in PD and reveal a precise interplay between DA and H 2 O 2 in the initiation of involuntary locomotion.