National Cancer Institute (NCI) Program for Natural Products Discovery: Rapid Isolation and Identification of Biologically Active Natural Products from the NCI Prefractionated Library | Zendy

Tanja Grkovic | Zendy; Rhone K. Akee | Zendy; Christopher C. Thornburg | Zendy; Spencer K. Trinh | Zendy; John R. Britt | Zendy; Matthew Harris | Zendy; Jason R. Evans | Zendy; Unwoo Kang | Zendy; Susan M. Ensel | Zendy; Curtis J. Henrich | Zendy; Kirk R. Gustafson | Zendy; Joel P. Schneider | Zendy; Barry R. O’Keefe | Zendy

Open Access

National Cancer Institute (NCI) Program for Natural Products Discovery: Rapid Isolation and Identification of Biologically Active Natural Products from the NCI Prefractionated Library

Author(s) -

Tanja Grkovic,

Rhone K. Akee,

Christopher C. Thornburg,

Spencer K. Trinh,

John R. Britt,

Matthew Harris,

Jason R. Evans,

Unwoo Kang,

Susan M. Ensel,

Curtis J. Henrich,

Kirk R. Gustafson,

Joel P. Schneider,

Barry R. O’Keefe

Publication year - 2020

Publication title -

acs chemical biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.899

H-Index - 111

eISSN - 1554-8937

pISSN - 1554-8929

DOI - 10.1021/acschembio.0c00139

Subject(s) - identification (biology) , biological activity , throughput , workflow , computational biology , chemistry , isolation (microbiology) , combinatorial chemistry , high throughput screening , high performance liquid chromatography , chromatography , computer science , biochemistry , biology , database , bioinformatics , in vitro , telecommunications , botany , wireless

An automated, high-capacity, and high-throughput procedure for the rapid isolation and identification of biologically active natural products from a prefractionated library is presented. The semipreparative HPLC method uses 1 mg of the primary hit fraction and produces 22 subfractions in an assay-ready format. Following screening, all active fractions are analyzed by NMR, LCMS, and FTIR, and the active principle structural classes are elucidated. In the proof-of-concept study, we show the processes involved in generating the subfractions, the throughput of the structural elucidation work, as well as the ability to rapidly isolate and identify new and biologically active natural products. Overall, the rapid second-stage purification conserves extract mass, requires much less chemist time, and introduces knowledge of structure early in the isolation workflow.

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