Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching | Zendy

Eljesa Murtezi | Zendy; Sujitha Puthukodan | Zendy; Jaroslaw Jacak | Zendy; Thomas A. Klar | Zendy

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Biofunctionalization of Sub-Diffractionally Patterned Polymer Structures by Photobleaching

Author(s) -

Eljesa Murtezi,

Sujitha Puthukodan,

Jaroslaw Jacak,

Thomas A. Klar

Publication year - 2018

Publication title -

acs applied materials and interfaces

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.535

H-Index - 228

eISSN - 1944-8252

pISSN - 1944-8244

DOI - 10.1021/acsami.8b11777

Subject(s) - sted microscopy , photobleaching , materials science , nanolithography , streptavidin , nanotechnology , stimulated emission , laser , fluorescence , optoelectronics , optics , chemistry , fabrication , medicine , biochemistry , physics , biotin , alternative medicine , pathology

Stimulated emission depletion (STED) nanolithography allows nanofabrication below the diffraction limit. Recently, it was applied to nanoanchors for protein fixation down to the single molecule level. We now combined STED nanolithography with laser-assisted protein adsorption by photobleaching (LAPAP) for optical and selective attachment of proteins to subdiffractional structures. In turn, STED was used for imaging of fluorescently tagged streptavidin to reveal protein binding to STED-lithographically patterned acrylate structures via LAPAP. Protein localization down to 56 nm spots was achieved using all-optical methods at visible wavelengths.

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