Open AccessPhotoperiodic Flower Mimicking Metallic Nanoparticles for Image-Guided Medicine Applications
Author(s) -
Soojeong Cho,
Byeongdu Lee,
Wooram Park,
Xiaoke Huang,
DongHyun Kim
Publication year - 2018
Publication title -
acs applied materials and interfaces
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.535
H-Index - 228
eISSN - 1944-8252
pISSN - 1944-8244
DOI - 10.1021/acsami.8b09596
Subject(s) - clonogenic assay , materials science , nanoparticle , reactive oxygen species , in vivo , apoptosis , nanotechnology , cancer cell , liver cancer , cancer research , irradiation , biophysics , cancer , biology , biochemistry , hepatocellular carcinoma , genetics , microbiology and biotechnology , physics , nuclear physics
Nanoradiosensitizers have been developed to enhance localization and precision of therapeutic radiation delivery. A specific volume of comprising surface atoms is known to be the radiosensitizing region. However, the shape-dependent local dose enhancement of nanoparticles is often underestimated and rarely reported. Here, a noble metal nanostructure, inspired by the photoperiodic day-flowers, was synthesized by metal reduction with bile acid molecules. The impact of high surface area of day-flower mimicking metallic nanoparticles (D-NP) on radiosensitizing effect was demonstrated with assays for ROS generation, cellular apoptosis, and clonogenic survival of human liver cancer cells (HepG2) cells. In comparison with lower-surface-area spherical night-flower mimicking metallic nanoparticles (N-NP), exposure of our D-NP to external beam radiation doses led to a significant increase in reactive oxygen species (ROS) production and radiosensitizing cell cycle synchronization, resulting in an enhanced cancer-cell-killing effect. In clonogenic survival studies, dose-enhancing factor (DEF) of D-NP was 16.5-fold higher than N-NP. Finally, we demonstrated in vivo feasibility of our D-NP as a potent nanoradiosensitizer and CT contrast agent for advanced image-guided radiation therapy.