Fast, Ultrasensitive Detection of Reactive Oxygen Species Using a Carbon Nanotube Based-Electrocatalytic Intracellular Sensor | Zendy

Frankie J. Rawson | Zendy; Jacqueline M. Hicks | Zendy; N.J.F. Dodd | Zendy; Wondwossen Abate | Zendy; David J. Garrett | Zendy; Nga Chi Yip | Zendy; György Fejér | Zendy; Alison J. Downard | Zendy; Keith Baronian | Zendy; Simon K. Jackson | Zendy; Paula M. Mendes | Zendy

Open Access

Fast, Ultrasensitive Detection of Reactive Oxygen Species Using a Carbon Nanotube Based-Electrocatalytic Intracellular Sensor

Author(s) -

Frankie J. Rawson,

Jacqueline M. Hicks,

N.J.F. Dodd,

Wondwossen Abate,

David J. Garrett,

Nga Chi Yip,

György Fejér,

Alison J. Downard,

Keith Baronian,

Simon K. Jackson,

Paula M. Mendes

Publication year - 2015

Publication title -

acs applied materials and interfaces

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.535

H-Index - 228

eISSN - 1944-8252

pISSN - 1944-8244

DOI - 10.1021/acsami.5b06493

Subject(s) - reactive oxygen species , intracellular , nadph oxidase , carbon nanotube , materials science , hydrogen peroxide , innate immune system , lipopolysaccharide , tlr4 , biophysics , electrocatalyst , nanotechnology , microbiology and biotechnology , electrode , electrochemistry , receptor , biochemistry , chemistry , signal transduction , biology , immunology

Herein, we report a highly sensitive electrocatalytic sensor-cell construct that can electrochemically communicate with the internal environment of immune cells (e.g., macrophages) via the selective monitoring of a particular reactive oxygen species (ROS), hydrogen peroxide. The sensor, which is based on vertically aligned single-walled carbon nanotubes functionalized with an osmium electrocatalyst, enabled the unprecedented detection of a local intracellular "pulse" of ROS on a short second time scale in response to bacterial endotoxin (lipopolysaccharide-LPS) stimulation. Our studies have shown that this initial pulse of ROS is dependent on NADPH oxidase (NOX) and toll like receptor 4 (TLR4). The results suggest that bacteria can induce a rapid intracellular pulse of ROS in macrophages that initiates the classical innate immune response of these cells to infection.

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