Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide | Zendy

Noelia Rubio | Zendy; KuoChing Mei | Zendy; Rebecca Klippstein | Zendy; Pedro M. Costa | Zendy; Naomi O. Hodgins | Zendy; Julie Wang | Zendy; Frederic Festy | Zendy; Vincenzo Abbate | Zendy; Robert C. Hider | Zendy; K. L. Andrew Chan | Zendy; Khuloud T. AlJamal | Zendy

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Solvent-Free Click-Mechanochemistry for the Preparation of Cancer Cell Targeting Graphene Oxide

Author(s) -

Noelia Rubio,

KuoChing Mei,

Rebecca Klippstein,

Pedro M. Costa,

Naomi O. Hodgins,

Julie Wang,

Frederic Festy,

Vincenzo Abbate,

Robert C. Hider,

K. L. Andrew Chan,

Khuloud T. AlJamal

Publication year - 2015

Publication title -

acs applied materials and interfaces

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.535

H-Index - 228

eISSN - 1944-8252

pISSN - 1944-8244

DOI - 10.1021/acsami.5b06250

Subject(s) - materials science , biocompatibility , click chemistry , polyethylene glycol , nuclear chemistry , polymer chemistry , organic chemistry , chemistry , metallurgy

Polyethylene glycol-functionalized nanographene oxide (PEGylated n-GO) was synthesized from alkyne-modified n-GO, using solvent-free click-mechanochemistry, i.e., copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). The modified n-GO was subsequently conjugated to a mucin 1 receptor immunoglobulin G antibody (anti-MUC1 IgG) via thiol-ene coupling reaction. n-GO derivatives were characterized with Fourier-transformed infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA), Bradford assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and atomic force microscopy (AFM). Cell targeting was confirmed in vitro in MDA-MB-231 cells, either expressing or lacking MUC1 receptors, using flow cytometry, confocal laser scanning microscopy (CLSM) and multiphoton (MP) fluorescence microscopy. Biocompatibility was assessed using the modified lactate dehydrongenase (mLDH) assay.

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