Open AccessVortex Fluidic-Mediated Fabrication of Fast Gelated Silica Hydrogels with Embedded Laccase Nanoflowers for Real-Time Biosensing under Flow
Author(s) -
Xuan Luo,
Ahmed Hussein Mohammed AlAntaki,
Aghil Igder,
Keith A. Stubbs,
Peng Su,
Wei Zhang,
Gregory A. Weiss,
Colin L. Raston
Publication year - 2020
Publication title -
acs applied materials and interfaces
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.535
H-Index - 228
eISSN - 1944-8252
pISSN - 1944-8244
DOI - 10.1021/acsami.0c15669
Subject(s) - materials science , fluidics , microfluidics , fabrication , chemical engineering , biosensor , coating , nanotechnology , catalysis , laccase , self healing hydrogels , organic chemistry , polymer chemistry , chemistry , medicine , alternative medicine , pathology , aerospace engineering , engineering , enzyme
The fabrication of hybrid protein-Cu 3 (PO 4 ) 2 nanoflowers (NFs) via an intermediate toroidal structure is dramatically accelerated under shear using a vortex fluidic device (VFD), which possesses a rapidly rotating angled tube. As-prepared laccase NFs (LNFs) exhibit ≈1.8-fold increase in catalytic activity compared to free laccase under diffusion control, which is further enhanced by ≈ 2.9-fold for the catalysis under shear in the VFD. A new LNF immobilization platform, LNF@silica incorporated in a VFD tube, was subsequently developed by mixing the LNFs for 15 min with silica hydrogel resulting in gelation along the VFD tube surface. The resulting LNFs@silica coating is highly stable and reusable, which allows a dramatic 16-fold enhancement in catalytic rates relative to LNF@silica inside glass vials. Ultraviolet-visible spectroscopy-based real-time monitoring within the LNFs@silica-coated tube reveals good stability of the coating in continuous flow processing. The results demonstrate the utility of the VFD microfluidic platform, further highlighting its ability to control chemical and enzymatic processes.