Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells? | Zendy

Levon Manukyan | Zendy; Maria-Eleni Marinaki | Zendy; Albert Mihranyan | Zendy

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Would 20 nm Filtered Fetal Bovine Serum-Supplemented Media Support Growth of CHO and HEK-293 Cells?

Author(s) -

Levon Manukyan,

Maria-Eleni Marinaki,

Albert Mihranyan

Publication year - 2020

Publication title -

acs applied bio materials

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.764

H-Index - 17

ISSN - 2576-6422

DOI - 10.1021/acsabm.0c01372

Subject(s) - chinese hamster ovary cell , fetal bovine serum , hek 293 cells , bovine serum albumin , cell culture , baby hamster kidney cell , cell , chemistry , biology , microbiology and biotechnology , andrology , immunology , biochemistry , medicine , genetics

Virus safety of fetal bovine serum (FBS) is a critical issue for cell culture and clinical applications of cell therapies. The size exclusion filtration of FBS-supplemented cell culture media through small-size virus retentive filter paper is presented to investigate its effect on cell culture. A substantial proportion of proteins (ca. 45%) was removed by nanofiltration, yet important transport proteins (albumin, fetuins, macroglobulins, transferrin) were unaffected. The cell viability of Chinese hamster ovary (CHO) and human embryonic kidney 293 (HEK-293) cells that were grown in media supplemented with nanofiltered FBS was surprisingly high, despite the observed protein losses. Protein depletion following nanofiltration resulted in detectable levels of autophagy markers.

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