Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants | Zendy

Kathryn A. Murray | Zendy; Ruben M. F. Tomás | Zendy; Matthew I. Gibson | Zendy

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Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants

Author(s) -

Kathryn A. Murray,

Ruben M. F. Tomás,

Matthew I. Gibson

Publication year - 2020

Publication title -

acs applied bio materials

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.764

H-Index - 17

ISSN - 2576-6422

DOI - 10.1021/acsabm.0c00638

Subject(s) - cryopreservation , cryoprotectant , mesenchymal stem cell , regenerative medicine , flow cytometry , stromal cell , microbiology and biotechnology , cell therapy , stem cell , chemistry , biology , immunology , cancer research , embryo

Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.

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