Open AccessCarbon as a Simple Support for Redox Biocatalysis in Continuous Flow
Author(s) -
Barnabas Poznansky,
Lisa A. Thompson,
Sarah A. Warren,
Holly A. Reeve,
Kylie A. Vincent
Publication year - 2020
Publication title -
organic process research and development
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 109
eISSN - 1520-586X
pISSN - 1083-6160
DOI - 10.1021/acs.oprd.9b00410
Subject(s) - biocatalysis , formate dehydrogenase , chemistry , cofactor , formate , space velocity , redox , methanol , lactate dehydrogenase , packed bed , catalysis , immobilized enzyme , combinatorial chemistry , organic chemistry , chromatography , enzyme , reaction mechanism , selectivity
A continuous packed bed reactor for NADH-dependent biocatalysis using enzymes co-immobilized on a simple carbon support was optimized to 100% conversion in a residence time of 30 min. Conversion of pyruvate to lactate was achieved by co-immobilized lactate dehydrogenase and formate dehydrogenase, providing in situ cofactor recycling. Other metrics were also considered as optimization targets, such as low E factors between 2.5-11 and space-time yields of up to 22.9 g L -1 h -1 . The long-term stability of the biocatalytic reactor was also demonstrated, with full conversion maintained over more than 30 h of continuous operation.
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