Open AccessSquarate Cross-Linked Gelatin Hydrogels as Three-Dimensional Scaffolds for Biomedical Applications
Author(s) -
Simone Stucchi,
Danilo Colombo,
Roberto Guizzardi,
Alessia D’Aloia,
Maddalena Collini,
Margaux Bouzin,
Barbara Costa,
Michela Ceriani,
Antonino Natalello,
Piersandro Pallavicini,
Laura Cipolla
Publication year - 2021
Publication title -
langmuir
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.042
H-Index - 333
eISSN - 1520-5827
pISSN - 0743-7463
DOI - 10.1021/acs.langmuir.1c02080
Subject(s) - self healing hydrogels , gelatin , swelling , chemistry , chemical engineering , chondrocyte , tissue engineering , scaffold , regenerative medicine , materials science , nanotechnology , polymer chemistry , cell , biomedical engineering , composite material , organic chemistry , biochemistry , medicine , engineering , in vitro
Hydrogels are useful platforms as three-dimensional (3D) scaffolds for cell culture, drug-release systems, and regenerative medicine applications. Here, we propose a novel chemical cross-linking approach by the use of 3,4-diethoxy-3-cyclobutene-1,2-dione or diethyl squarate for the preparation of 5 and 10% w/v gelatin-based hydrogels. Hydrogels showed good swelling properties, and the 5% gelatin-based hydrogel proved suitable as a 3D cell culture scaffold for the chondrocyte cell line C28/I2. In addition, diffusion properties of different sized molecules inside the hydrogel were determined.