Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord | Zendy

Luis Felipe Clemente | Zendy; María Luisa Hernáez | Zendy; António Ramos-Fernández | Zendy; Gertrudis Ligero | Zendy; Concha Gil | Zendy; Fernando J. Corrales | Zendy; Miguel Marcilla | Zendy

Open Access

Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord

Author(s) -

Luis Felipe Clemente,

María Luisa Hernáez,

António Ramos-Fernández,

Gertrudis Ligero,

Concha Gil,

Fernando J. Corrales,

Miguel Marcilla

Publication year - 2018

Publication title -

journal of proteome research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.644

H-Index - 161

eISSN - 1535-3907

pISSN - 1535-3893

DOI - 10.1021/acs.jproteome.8b00384

Subject(s) - mesenchymal stem cell , adipose tissue , proteomics , proteome , biology , stem cell , microbiology and biotechnology , umbilical cord , biochemistry , immunology , gene

Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labeled peptides that coeluted with their endogenous light counterparts. Our data also suggest that mesenchymal stem cells constitute a promising source for the detection of missing proteins.

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