Synthesis of 4-Azido-N-acetylhexosamine Uridine Diphosphate Donors: Clickable Glycosaminoglycans | Zendy

Xing Zhang | Zendy; Dixy E. Green | Zendy; Victor Schultz | Zendy; Lei Lin | Zendy; Xiaorui Han | Zendy; Ruitong Wang | Zendy; Alexander Yaksic | Zendy; So Young Kim | Zendy; Paul L. DeAngelis | Zendy; Robert J. Linhardt | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Synthesis of 4-Azido-N-acetylhexosamine Uridine Diphosphate Donors: Clickable Glycosaminoglycans

Author(s) -

Xing Zhang,

Dixy E. Green,

Victor Schultz,

Lei Lin,

Xiaorui Han,

Ruitong Wang,

Alexander Yaksic,

So Young Kim,

Paul L. DeAngelis,

Robert J. Linhardt

Publication year - 2017

Publication title -

the journal of organic chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.2

H-Index - 228

eISSN - 1520-6904

pISSN - 0022-3263

DOI - 10.1021/acs.joc.7b01787

Subject(s) - chemistry , glycosaminoglycan , uridine diphosphate , uridine , uridine diphosphate glucose , nucleotide sugar , alexa fluor , nucleotide , alkyne , chondroitin , substrate (aquarium) , stereochemistry , biochemistry , polysaccharide , rna , enzyme , fluorescence , physics , quantum mechanics , gene , catalysis , oceanography , geology

Unnatural chemically modified nucleotide sugars UDP-4-N 3 -GlcNAc and UDP-4-N 3 -GalNAc were chemically synthesized for the first time. These unnatural UDP sugar products were then tested for incorporation into hyaluronan, heparosan, or chondroitin using polysaccharide synthases. UDP-4-N 3 -GlcNAc served as a chain termination substrate for hyaluronan or heparosan synthases; the resulting 4-N 3 -GlcNAc-terminated hyaluronan and heparosan were then successfully conjugated with Alexa Fluor 488 DIBO alkyne, demonstrating that this approach is generally applicable for labeling and detection of suitable glycosaminoglycans.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research