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Development of Betalain Producing Callus Lines from Colored Quinoa Varieties (Chenopodium quinoa Willd)
Author(s) -
Paula HenarejosEscudero,
Berenice Guadarrama-Flores,
M. Alejandra GuerreroRubio,
Luz GomezPando,
Francisco Garcı́a-Carmona,
Fernando GandíaHerrero
Publication year - 2017
Publication title -
journal of agricultural and food chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.203
H-Index - 297
eISSN - 1520-5118
pISSN - 0021-8561
DOI - 10.1021/acs.jafc.7b04642
Subject(s) - chenopodium quinoa , betalain , callus , pigment , botany , biological pigment , colored , chenopodium , amaranthaceae , biology , chemistry , food science , horticulture , carotenoid , materials science , weed , organic chemistry , composite material
Betalains are water-soluble plant pigments of hydrophilic nature with promising bioactive potential. Among the scarce edible sources of betalains is the grain crop quinoa (Chenopodium quinoa Willd), with violet, red, and yellow grains being colored by these pigments. In this work, callus cultures have been developed from differently colored plant varieties. Stable callus lines exhibited color and pigment production when maintained on Murashige and Skoog medium supplemented with the plant growth regulators 6-benzylaminopurine (8.88 μM) and 2,4-dichlorophenoxyacetic acid (6.79 μM) with a reduction of the nitrogen source to 5.91 mM. Pigment analysis by HPLC-DAD and ESI-MS/MS fully describes the content of individual pigments in the cell lines and allows the first report on the pigments present in quinoa seedlings. Phyllocactin and vulgaxanthin I are described as novel pigments in the species and show the potential of C. quinoa culture lines in the production of compounds of nutritional value.

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