Antioxidant Mechanism of Betaine without Free Radical Scavenging Ability | Zendy

Mengmeng Zhang | Zendy; Hong Zhang | Zendy; Huixian Li | Zendy; Furao Lai | Zendy; Xiaofeng Li | Zendy; Yuqian Tang | Zendy; Min Tian | Zendy; Hui Wu | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Antioxidant Mechanism of Betaine without Free Radical Scavenging Ability

Author(s) -

Mengmeng Zhang,

Hong Zhang,

Huixian Li,

Furao Lai,

Xiaofeng Li,

Yuqian Tang,

Min Tian,

Hui Wu

Publication year - 2016

Publication title -

journal of agricultural and food chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.203

H-Index - 297

eISSN - 1520-5118

pISSN - 0021-8561

DOI - 10.1021/acs.jafc.6b03592

Subject(s) - scavenging , betaine , antioxidant , chemistry , mechanism (biology) , radical , biochemistry , food science , philosophy , epistemology

Betaine (BET) is a native compound widely studied as an antioxidant in agriculture and human health. However, the antioxidant mechanism of BET remains unclear. In this research, radical scavenging assays showed that BET had little free radical scavenging activity. However, the antioxidant activity of BET was confirmed by cellular antioxidant activity (CAA) and erythrocyte hemolysis assays. The results of quantitative PCR (qPCR) and enzyme activity determination kits showed that the antioxidant activity of BET was not due to the gene expression and activity of antioxidases. High-pressure liquid chromatography (HPLC) assessment of the effect of BET on sulfur-containing amino acid metabolism showed that BET increased the levels of nonenzymatic antioxidants,S-adenosylmethionine (SAM) and methionine (p < 0.05), via the regulation of the methionine-omocysteine cycle. Additionally, the three methyl groups of BET were found to play a key role in its antioxidant activity. The possible reason was that because of the hydrophobicity of the three methyl groups and hydrophilicity of the carboxyl of BET, a tight protective membrane was formed around cells to prevent oxidative stress inducer from inducing ROS generation and cell damage. In conclusion, the antioxidant mechanism of BET was found to enhance nonenzymatic antioxidant defenses via the methionine-homocysteine cycle and form a protective membrane around cells.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research