Identification of New Antimicrobial Peptides that Contribute to the Bactericidal Activity of Egg White against Salmonella enterica Serovar Enteritidis at 45 °C | Zendy

MarieFrançoise Cochet | Zendy; Florence Baron | Zendy; Sylvie Bonnassié | Zendy; Sophie Jan | Zendy; Nadine Leconte | Zendy; Julien Jardin | Zendy; Valérie BriardBion | Zendy; Michel Gautier | Zendy; Simon C. Andrews | Zendy; Catherine GuérinDubiard | Zendy; Françoise Nau | Zendy

Open Access

Identification of New Antimicrobial Peptides that Contribute to the Bactericidal Activity of Egg White against Salmonella enterica Serovar Enteritidis at 45 °C

Author(s) -

MarieFrançoise Cochet,

Florence Baron,

Sylvie Bonnassié,

Sophie Jan,

Nadine Leconte,

Julien Jardin,

Valérie BriardBion,

Michel Gautier,

Simon C. Andrews,

Catherine GuérinDubiard,

Françoise Nau

Publication year - 2021

Publication title -

journal of agricultural and food chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.203

H-Index - 297

eISSN - 1520-5118

pISSN - 0021-8561

DOI - 10.1021/acs.jafc.0c06677

Subject(s) - antimicrobial , salmonella enterica , salmonella enteritidis , antimicrobial peptides , serotype , microbiology and biotechnology , chemistry , egg white , salmonella , ultrafiltration (renal) , food science , peptide , escherichia coli , biology , chromatography , bacteria , biochemistry , genetics , gene

A recent work revealed that egg white (EW) at 45 °C exhibits powerful bactericidal activity agains S. enterica serovar Enteritidis, which is surprisingly little affected by removal of the >10 kDa EW proteins. Here, we sought to identify the major EW factors responsible for this bactericidal activity by fractionating EW using ultrafiltration and nanofiltration and by characterizing the physicochemical and antimicrobial properties of the resulting fractions. In particular, 22 peptides were identified by nano-LC/MS-MS and the bactericidal activities of representative peptides (with predicted antimicrobial activity) were further assessed. Two peptides (FVPPVQR and GDPSAWSWGAEAHS) were found to be bactericidal agains S. enterica serovar Enteritidis at 45 °C when provided in an EW environment. Nevertheless, these peptides contribute only part of this bactericidal activity, suggesting other, yet to be determined, antimicrobial factors.

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