Evidence of an Unusual Poly(A) RNA Signature Detected by High-Throughput Chemical Mapping | Zendy

Roger Wellington-Oguri | Zendy; Eli Fisker | Zendy; Mathew Zada | Zendy; Michelle Wiley | Zendy; Jill Townley | Zendy; Eterna Players | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Evidence of an Unusual Poly(A) RNA Signature Detected by High-Throughput Chemical Mapping

Author(s) -

Roger Wellington-Oguri,

Eli Fisker,

Mathew Zada,

Michelle Wiley,

Jill Townley,

Eterna Players

Publication year - 2020

Publication title -

biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.43

H-Index - 253

eISSN - 1520-4995

pISSN - 0006-2960

DOI - 10.1021/acs.biochem.0c00215

Subject(s) - primer extension , signature (topology) , nucleotide , acylation , rna , substitution (logic) , chemistry , sequence (biology) , stereochemistry , computational biology , biology , biochemistry , microbiology and biotechnology , gene , computer science , geometry , mathematics , programming language , catalysis

Homopolymeric adenosine RNA plays numerous roles in both cells and noncellular genetic material. We report herein an unusual poly(A) signature in chemical mapping data generated by the Eterna Massive Open Laboratory. Poly(A) sequences of length seven or more show unexpected results in the selective 2'-hydroxyl acylation read out by primer extension (SHAPE) and dimethyl sulfate (DMS) chemical probing. This unusual signature first appears in poly(A) sequences of length seven and grows to its maximum strength at length ∼10. In a long poly(A) sequence, the substitution of a single A by any other nucleotide disrupts the signature, but only for the 6 or so nucleotides on the 5' side of the substitution.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research