Single-Nanoparticle ICPMS DNA Assay Based on Hybridization-Chain-Reaction-Mediated Spherical Nucleic Acid Assembly | Zendy

Bang-Rui Li | Zendy; Hao Tang | Zendy; RuQin Yu | Zendy; JianHui Jiang | Zendy

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Single-Nanoparticle ICPMS DNA Assay Based on Hybridization-Chain-Reaction-Mediated Spherical Nucleic Acid Assembly

Author(s) -

Bang-Rui Li,

Hao Tang,

RuQin Yu,

JianHui Jiang

Publication year - 2020

Publication title -

analytical chemistry

Language(s) - Uncategorized

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.9b05741

Subject(s) - nucleic acid , chemistry , nucleic acid quantitation , dna , biomolecule , nucleic acid thermodynamics , nanoparticle , colloidal gold , mass spectrometry , nanotechnology , chromatography , biochemistry , base sequence , materials science

The detection of nucleic acid is critical for clinic diagnostics. Single-nanoparticle inductively coupled plasma mass spectrometry (SP-ICPMS) has demonstrated unique advantages for nucleic acid detection. Here we report the development of a novel SP-ICPMS DNA assay based on a target-induced hybridization chain reaction to achieve controlled spherical nucleic acid assembly. The assembly process generated large gold nanoparticle aggregates, and the number of aggregates could be counted by SP-ICPMS, which was closely correlated to the concentration of the target DNA. This simple homogeneous assay could analyze DNA within the range of 5 fM to 10 pM with excellent selectivity and applicability for real sample analysis. It is envisioned that the developed approach might create a useful SP-ICPMS platform for biomolecule detection.

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