Portable and Field-Ready Detection of Circulating MicroRNAs with Paper-Based Bioluminescent Sensing and Isothermal Amplification | Zendy

Yong Li | Zendy; Lanlan Zhou | Zendy; Wei Ni | Zendy; Qingying Luo | Zendy; Chunnan Zhu | Zendy; Yunhua Wu | Zendy

Open Access

Portable and Field-Ready Detection of Circulating MicroRNAs with Paper-Based Bioluminescent Sensing and Isothermal Amplification

Author(s) -

Yong Li,

Lanlan Zhou,

Wei Ni,

Qingying Luo,

Chunnan Zhu,

Yunhua Wu

Publication year - 2019

Publication title -

analytical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.117

H-Index - 332

eISSN - 1520-6882

pISSN - 0003-2700

DOI - 10.1021/acs.analchem.9b04422

Subject(s) - loop mediated isothermal amplification , bioluminescence , chemistry , microrna , isothermal process , software deployment , field (mathematics) , energy transfer , nanotechnology , computer science , biochemistry , dna , physics , materials science , mathematics , molecular physics , pure mathematics , gene , operating system , thermodynamics

We present a paper-based system that integrates bioluminescence resonance energy transfer (BRET) and isothermal amplification for the analysis of tumor-associated circulating microRNAs (miRNAs) in clinical serum samples. The analysis procedure could be easily accomplished with two pieces of functionalized paper and a low-cost smartphone-based device, which enables sequence-specific quantification of femtomolar miRNAs, without the need for tedious handling of aqueous reactions and operation of sophisticated equipment. Furthermore, the analytical performance of the proposed paper-based system was highly stable at room temperature, demonstrating its capability for cold-chain-free and remote deployment. These qualities highlight the practical utility of our method for the portable and field-ready miRNA diagnostic tests in resource-limited settings.

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